PXD038501

PXD038501 is an original dataset announced via ProteomeXchange.

Title	High-throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags
Description	Glycans, which are widely distributed on most proteins and cell surfaces, are a class of important biomolecules playing important roles in various biological processes such as immune response and cellular communication. Modern mass spectrometry (MS) and novel chemical probes together greatly facilitate the routine analysis of glycans. However, the requirement of high-throughput analysis still calls for advanced tools to be developed. Recently, we devised isobaric multiplex reagents for carbonyl-containing compound (SUGAR) tags for N-glycans analysis capable of processing four samples at a time. To further improve the throughput, we utilized the subtle mass differences among different isotopologues combinations and expanded the multiplexing capacity to twelve channels, a three-fold throughput improvement compared to the original setup and achieved ultra-high throughput N-glycans analysis in a single LC-MS/MS injection. We then applied 12-plex SUGAR tags to profile the N-glycans in four subtypes of human Immunoglobulin G (IgG) and to investigate the N-glycans changes in the endometrial cancer cells (ECC1) treated with Atovaquone, a quinone antimicrobial medication, and a dihydroorotate dehydrogenase (DHODH) inhibitor.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:27.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Ma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-01 04:41:33	ID requested
1	2023-03-11 10:27:18	announced
⏵ 2	2023-11-14 08:43:29	announced	2023-11-14: Updated project metadata.

Li M, Feng Y, Ma M, Kapur A, Patankar M, Li L, High-Throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags. J Proteome Res, 22(5):1557-1563(2023) [pubmed]

submitter keyword: N-glycan,Lc-MS/MS,isobaric labeling

lingjun Li
contact affiliation	School of Pharmacy University of Wisconsin 777 Highland Ave. Madison, WI 53705-2222
contact email	lingjun.li@wisc.edu
lab head
Min Ma
contact affiliation	UW madison
contact email	mma58@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038501

PRIDE project URI

• PRIDE
  1. PXD038501
     1. Label: PRIDE project
     2. Name: High-throughput Quantitative Glycomics Enabled by 12-plex Isobaric Multiplex Labeling Reagents for Carbonyl-Containing Compound (SUGAR) Tags