PXD038480

PXD038480 is an original dataset announced via ProteomeXchange.

Title	Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light
Description	Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. However, the cellular mechanism underlying ROS-induced leaf damage alleviation in peroxisomes was not fully explored. Here, we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We present that autophagy-deficient mutants show light intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates are specifically engulfed by pre-autophagosomal structures and vacuolar membranes in both leaf cells and isolated vacuoles, but they are not degraded in mutants. ATG18a-GFP and GFP-2×FYVE, which bind to phosphatidylinositol 3-phosphate, preferentially target the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulating cells under high-intensity light. Our findings provide deeper insights into the plant stress response caused by light irradiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:33:37.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kenji Yamada
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-01 02:11:56	ID requested
1	2023-02-27 01:14:25	announced
⏵ 2	2023-11-14 08:33:38	announced	2023-11-14: Updated project metadata.

Oikawa K, Goto-Yamada S, Hayashi Y, Takahashi D, Kimori Y, Shibata M, Yoshimoto K, Takemiya A, Kondo M, Hikino K, Kato A, Shimoda K, Ueda H, Uemura M, Numata K, Ohsumi Y, Hara-Nishimura I, Mano S, Yamada K, Nishimura M, Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light. Nat Commun, 13(1):7493(2022) [pubmed]

submitter keyword: LC-MSMS, Peroxisome, Autophagy,Arabitopsis thaliana

Kenji Yamada
contact affiliation	Plant Molecular Biology Lab, Malopolska Centre of Biotechnology, Jagiellonian University
contact email	kenji.yamada@uj.edu.pl
lab head
Kenji Yamada
contact affiliation	Jagiellonian University
contact email	kenji.yamada@uj.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038480
     1. Label: PRIDE project
     2. Name: Pexophagy suppresses ROS-induced damage in leaf cells under high-intensity light