PXD038478

PXD038478 is an original dataset announced via ProteomeXchange.

Title	The role of RNF149 in the pre-emptive quality control substrate ubiquitination
Description	Protein quality control is a continuous process in which a protein's folding status is constantly monitored. Mislocalized proteins (MLP), are processed by the various quality control pathways, as they are often misfolded due to inappropriate cellular surroundings. Polypeptides that fail to translocate into the ER due to an inefficient signal peptide, mutations or ER stress are recognized by the pre-emptive ER associated quality control (pEQC) pathway and degraded by the 26S proteasome. In this report we reveal the role of RNF149, a membrane bound E3 ligase in the ubiquitination of known pEQC substrates. We demonstrate its selective binding only to non-translocated proteins and its association with known pEQC components. Impairment in RNF149 function increases translocation flux into the ER and manifests in a myeloproliferative neoplasm (MPN) phenotype, a pathological condition associated with pEQC impairment. Finally, the dynamic localization of RNF149 may provides a molecular switch to regulate pEQC during ER stress. This project is lead by Ariel Stanhill (ariel.stanhill@gmail.com,Department of Natural and Life Sciences, The Open University of Israel, Raanana, Israel)
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:50.192.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038478
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Meital Kupervaser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-01 01:40:53	ID requested
1	2023-04-08 23:13:06	announced
2	2023-05-02 06:45:26	announced	2023-05-02: Updated project metadata.
⏵ 3	2023-11-14 08:59:50	announced	2023-11-14: Updated project metadata.

10.6019/PXD038478;

submitter keyword: pre-emptive ER associated quality control, membrane translocation, proteasome, RNF149

Ami Navon
contact affiliation	Department of Immunology and Regenerative Biology, Faculty of Biology, The Weizmann Institute of Science, Rehovot, Israel
contact email	ami.navon@weizmann.ac.il
lab head
Meital Kupervaser
contact affiliation	Weizmann Institute of Science
contact email	meital.kupervaser@weizmann.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038478
     1. Label: PRIDE project
     2. Name: The role of RNF149 in the pre-emptive quality control substrate ubiquitination