PXD038474

PXD038474 is an original dataset announced via ProteomeXchange.

Title	Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits
Description	Pol II has been recognized as a passively regulated holoenzyme. However, whether Pol II plays specific regulatory roles remain unclear. Here, fractions containing disassociated RPB3 (dRPB3) were identified by size exclusion chromatography in various cells. Through a unique strategy, Specific Degradation of Disassociated Subunits (SDDS), we demonstrated that dRPB3 functions as a regulatory component of Pol II to enable the preferential control of 3’ end processing of ribosomal protein genes directly through its N-terminal domain. Machine learning analysis of large-scale genomic features revealed that the little elongation complex helps to specialize the functions of dRPB3. Mechanistically, dRPB3 facilitates CBC-PCF11 axis activity to increase the efficiency of 3’ end processing. Furthermore, RPB3 is dynamically regulated during development and diseases. These findings suggest that Pol II gains specific regulatory functions by trapping disassociated subunits.
HostingRepository	PRIDE
AnnounceDate	2023-02-14
AnnouncementXML	Submission_2023-02-13_20:38:42.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wenjiaduan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-12-01 01:38:14	ID requested
⏵ 1	2023-02-13 20:38:42	announced

Dataset with its publication pending

submitter keyword: HEK293T RPB3 PTM

XiongJi
contact affiliation	Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
contact email	xiongji@pku.edu.cn
lab head
wenjiaduan
contact affiliation	Peking University
contact email	dwj960528@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038474
     1. Label: PRIDE project
     2. Name: Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits