PXD038466

PXD038466 is an original dataset announced via ProteomeXchange.

Title	PRM quantification of MCS proteins and viral proteins in HCMV virus microenvironment
Description	The control of host processes which influence cell-cell communication is central to determining the outcome of a virus infection in tissue. Despite this, it remains unclear how cells either in close or distant proximity to an infected cell differentially respond to the primary infection. We established a virus infection microenvironment to resolve molecular features and functional consequences of cell spatial address within this localized niche by proteomic analyses, and identified differential regulations of membrane contact site (MCS) proteins in different cell populations. Here, we conducted targeted mass spectrometry (PRM) analyses for all MCS proteins representing every major organelle-organelle association, and identified elevation of MCS proteins in infected and neighboring cells. To check the contribution of extracellular vesicle (EV) dependent mechanism to the viral proteins detected in the neighboring cells, HCMV protein PRM analyses were conducted with or without EV inhibitors, GW4869 or manumycin A (MA), showing that EVs contribute to the viral protein levels in neighboring population.
HostingRepository	PanoramaPublic
AnnounceDate	2023-05-22
AnnouncementXML	Submission_2023-05-22_20:21:59.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bokai Song
SpeciesList	scientific name: Human betaherpesvirus 5; NCBI TaxID: 10359; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-30 12:40:28	ID requested
1	2023-05-16 08:05:06	announced
⏵ 2	2023-05-22 20:22:00	announced	: Added PubMed Id

Song B, Sheng X, Justice JL, Lum KK, Metzger PJ, Cook KC, Kostas JC, Cristea IM, Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections. Sci Adv, 9(19):eadg3433(2023) [pubmed]

submitter keyword: virus microenvironment, HCMV, membrane contact site, PRM

Ileana Cristea
contact affiliation	Princeton University
contact email	icristea@princeton.edu
lab head
Bokai Song
contact affiliation	Princeton University
contact email	bokais@princeton.edu
dataset submitter

Panorama Public dataset URI