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PXD038439

PXD038439 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative analysis of the impact of CX-5461 on the proteome of Pancreatic ductal adenocarcinoma cells.
DescriptionTo reveal the effect of CX-5461 (a small-molecule inhibitor of ribosome biogenesis)on the proteome of Pancreatic Ductal Adenocarcinoma (PDAC) cells, we carried out two quantitative proteomics analyses, using tumour cells isolated from an inducible mouse model of PDAC (iKras PDAC) (Ying et al., 2012). In this model, oncogenic Kras (G12D) expression can be controlled by administration of doxycycline (Dox). In the first experiment, Tandem Mass Tagging (TMT) was employed for quantitative analysis of mock vs. CX-5461 (100nM) treated iKras cells for 48 hrs, in presence of Dox (Kras on). In the second experiment, TMT was used to quantitatively analyse the proteomics changes induced by Dox removal (48 hrs), in presence or absence of CX-5461. For this purpose, iKras PDAC cells were seeded and grown for 48 hrs with or without Dox, in the background of mock vs. CX-5461 (100nM) co-treatments. TMT labelling was done using the TMT 6plex labelling kit from Thermo Fisher.
HostingRepositoryPRIDE
AnnounceDate2023-02-12
AnnouncementXMLSubmission_2023-02-12_07:54:38.772.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFarazMardakheh
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-27 01:16:59ID requested
12023-02-12 07:54:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PDAC
KRAS
CX-5461
TMT;
Contact List
Faraz K.Mardakheh
contact affiliationCentre for Cancer Cell & Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK.
contact emailf.mardakheh@qmul.ac.uk
lab head
FarazMardakheh
contact affiliationBarts Cancer Institute
contact emailf.mardakheh@qmul.ac.uk
dataset submitter
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Dataset FTP location
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