⮝ Full datasets listing

PXD038428

PXD038428 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNutrient regulated control of lysosome function by signaling lipid conversion
DescriptionLysosomes serve dual antagonistic functions in cells by mediating anabolic growth signaling and the catabolic turnover of macromolecules. How these janus-faced activities are regulated in response to cellular nutrient status is poorly understood. We show here that lysosome morphology and function are reversibly controlled by a nutrient-regulated signaling lipid switch that triggers the conversion between motile mTOR complex 1 (mTORC1) signaling-active and static mTORC1-inactive degradative lysosomes clustered at the cell center. Starvation-triggered relocalization of phosphatidylinositol 4-phosphate [PI(4)P] metabolizing enzymes reshapes the lysosomal surface proteome to facilitate lysosomal proteolysis and to repress mTORC1-signaling by erasing phosphatidylinositol 3-phosphate [PI(3)P], which marks motile signaling-active lysosomes in the cell periphery. Interference with this PI(3)P/ PI(4)P lipid switch module impairs the adaptive response of cells to altering nutrient supply. Our data unravel a key function for lysosomal phosphoinositide metabolism in rewiring organellar membrane dynamics in response to cellular nutrient status.
HostingRepositoryPRIDE
AnnounceDate2025-02-10
AnnouncementXMLSubmission_2025-02-10_04:59:28.666.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPathma Muthukottiappan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-26 23:22:15ID requested
12025-02-10 04:59:29announced
Publication List
10.1016/J.CELL.2023.09.027;
Keyword List
submitter keyword: mTORC1,Lysosome, PI4K2A
Contact List
Dr.Dominic Winter
contact affiliationInstitute for Biochemistry and Molecular Biology, University of Bonn
contact emaildwin@uni-bonn.de
lab head
Pathma Muthukottiappan
contact affiliationInstitute for Biochemistry and Molecular Biology, University of Bonn
contact emailpamut@uni-bonn.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD038428
PRIDE project URI
Repository Record List
[ + ]