PXD038428

PXD038428 is an original dataset announced via ProteomeXchange.

Title	Nutrient regulated control of lysosome function by signaling lipid conversion
Description	Lysosomes serve dual antagonistic functions in cells by mediating anabolic growth signaling and the catabolic turnover of macromolecules. How these janus-faced activities are regulated in response to cellular nutrient status is poorly understood. We show here that lysosome morphology and function are reversibly controlled by a nutrient-regulated signaling lipid switch that triggers the conversion between motile mTOR complex 1 (mTORC1) signaling-active and static mTORC1-inactive degradative lysosomes clustered at the cell center. Starvation-triggered relocalization of phosphatidylinositol 4-phosphate [PI(4)P] metabolizing enzymes reshapes the lysosomal surface proteome to facilitate lysosomal proteolysis and to repress mTORC1-signaling by erasing phosphatidylinositol 3-phosphate [PI(3)P], which marks motile signaling-active lysosomes in the cell periphery. Interference with this PI(3)P/ PI(4)P lipid switch module impairs the adaptive response of cells to altering nutrient supply. Our data unravel a key function for lysosomal phosphoinositide metabolism in rewiring organellar membrane dynamics in response to cellular nutrient status.
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-10_04:59:28.666.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pathma Muthukottiappan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 23:22:15	ID requested
⏵ 1	2025-02-10 04:59:29	announced

10.1016/J.CELL.2023.09.027;

submitter keyword: mTORC1,Lysosome, PI4K2A

Dr.Dominic Winter
contact affiliation	Institute for Biochemistry and Molecular Biology, University of Bonn
contact email	dwin@uni-bonn.de
lab head
Pathma Muthukottiappan
contact affiliation	Institute for Biochemistry and Molecular Biology, University of Bonn
contact email	pamut@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038428
     1. Label: PRIDE project
     2. Name: Nutrient regulated control of lysosome function by signaling lipid conversion