PXD038414

PXD038414 is an original dataset announced via ProteomeXchange.

Title	Peptide-mediated inhibition of the transcriptional regulator Elongin BC induces apoptosis in cancer cells
Description	Inhibiting protein-protein interactions via designed peptides is a suitable strategy to block the function of important proteins in cells. The Elongin BC heterodimer (ELOB/C) is involved in transcription elongation and protein turnover. It interacts with target proteins via a so-called BC-box to modulate their functions. ELOB and ELOC are commonly upregulated in cancer and are essential for cancer cell growth, making them attractive drug targets. However, currently, no strategy has been established to inhibit the function of ELOB/C in cells. Here, we show that peptides that mimic the high-affinity BC-box of EPOP can block the interaction of ELOB/C with its target proteins, both in vitro and in the cellular environment. Cancer cells treated with BC-box peptides, but not with the mutated control, show decreased cell viability, altered cell cycle and increased apoptosis, demonstrating a biological impact by inhibiting ELOB/C in vivo. Together our work suggests that targeting the BC-box binding pocket of ELOB/C is a feasible strategy to impair the function of the ELOB/C heterodimer and to inhibit cancer cell growth.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:47.634.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Liefke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 05:31:40	ID requested
1	2023-06-27 13:16:47	announced
⏵ 2	2023-11-14 09:01:49	announced	2023-11-14: Updated project metadata.

Fischer S, Trinh VT, Simon C, Weber LM, Forn, é I, Nist A, Bange G, Abendroth F, Stiewe T, Steinchen W, Liefke R, V, á, zquez O, Peptide-mediated inhibition of the transcriptional regulator Elongin BC induces apoptosis in cancer cells. Cell Chem Biol, 30(7):766-779.e11(2023) [pubmed]

submitter keyword: Peptide Inhibition, Elongin BC, Cancer, PRC2,EPOP

Robert Liefke
contact affiliation	Institute of Molecular Biology and Tumor Research (IMT), Philipps University of Marburg, Marburg, 35043, Germany
contact email	robert.liefke@imt.uni-marburg.de
lab head
Robert Liefke
contact affiliation	University Marburg
contact email	robert.liefke@imt.uni-marburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD038414

PRIDE project URI

• PRIDE
  1. PXD038414
     1. Label: PRIDE project
     2. Name: Peptide-mediated inhibition of the transcriptional regulator Elongin BC induces apoptosis in cancer cells