PXD038411

PXD038411 is an original dataset announced via ProteomeXchange.

Title	Identification of Ubiquitination site of PAICS by LC-MS/MS
Description	Purines serve as the building blocks for DNA and RNA, confer cellular energy and signaling. Purines are generated by de novo synthesis pathway and salvage pathway. Under purine-depleted or other cellular stresses, enzymes in the purine de novo synthesis pathway form a dynamic and reversible condensate called purinosome, but the underlying mechanism of purinosome formation is unknown. In this thesis, we found that ASB11-based Cul5 E3 ligase promotes ubiquitination of PAICS, a purine de novo synthesis enzyme. This ubiquitination does not lead to PAICS degradation, but drives purinosome assembly. We provide evidence that purinosome assembly involves a liquid-liquid phase separation (LLPS) process and identify several ubiquitin binding proteins that may bind ubiquitinated PAICS through a multivalent mode to drive LLPS and purinosome assembly. Importantly, ASB11 is upregulated under the stresses that promote purinosome assembly, thus increasing the formation of ASB11/PAICS complex. Finally, we demonstrated that melanoma cells express a high level of ASB11 to confer a constitutive purinosome formation, which support their viability. In summary, our study identifies ASB11-mediated PAICS ubiquitination as a driving mechanism for purinosome assembly, the regulation of this mechanism under stressed conditions, and the importance of this regulation in cell viability.
HostingRepository	PRIDE
AnnounceDate	2023-10-06
AnnouncementXML	Submission_2023-10-06_05:38:02.340.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ming-ChiehCHOU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 05:30:33	ID requested
⏵ 1	2023-10-06 05:38:02	announced

Dataset with its publication pending

submitter keyword: Ubiquitination site, PAICS,ASB11

Ruey-HwaChen
contact affiliation	IBC R602,128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
contact email	rhchen@gate.sinica.edu.tw
lab head
Ming-ChiehCHOU
contact affiliation	Academia Sinica
contact email	mcchou0517@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038411

PRIDE project URI

• PRIDE
  1. PXD038411
     1. Label: PRIDE project
     2. Name: Identification of Ubiquitination site of PAICS by LC-MS/MS