PXD038410

PXD038410 is an original dataset announced via ProteomeXchange.

Title	Dehalobacterium formicoaceticum proteomics data from dichloromethane (DCM)-only medium
Description	Dehalobacterium formicoaceticum is recognised for its unusual ability to anaerobically ferment dichloromethane (DCM), and a catabolic model has recently been proposed. D. formicoaceticum is currently the only axenic culture representative of its class, the Dehalobacteriia, however much diversity has been revealed in this lineage through recent culture-independent exploration of anoxic habitats. Here we performed a comparative analysis of 10 members of the Dehalobacteriia, representing three orders, and infer that anaerobic DCM degradation is a recently acquired trait only present in some members of the order Dehalobacteriales. Inferred traits common to the class include the use of amino acids as sole carbon sources for growth, energy generation via a remarkable range of putative electron-bifurcating protein complexes, and S-layers. The ability of D. formicoaceticum to grow on serine alone was subsequently confirmed experimentally and a high abundance of the electron-bifurcating protein complexes and S-layer proteins was noted when this organism was grown on DCM. We conclude that members of the Dehalobacteriia are low abundance fermentative scavengers in anoxic habitats.
HostingRepository	PRIDE
AnnounceDate	2023-04-13
AnnouncementXML	Submission_2023-04-13_09:42:56.585.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YoungSong
SpeciesList	scientific name: Dehalobacterium formicoaceticum; NCBI TaxID: 51515;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 05:25:57	ID requested
⏵ 1	2023-04-13 09:42:56	announced

Dataset with its publication pending

submitter keyword: Dehalobacterium, dichloromethane,microbiology

PhilipHugenholtz
contact affiliation	Australian Centre for Ecogenomics, The University of Queensland
contact email	p.hugenholtz@uq.edu.au
lab head
YoungSong
contact affiliation	The University of Queensland
contact email	young.song@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD038410

PRIDE project URI

• PRIDE
  1. PXD038410
     1. Label: PRIDE project
     2. Name: Dehalobacterium formicoaceticum proteomics data from dichloromethane (DCM)-only medium