PXD038403

PXD038403 is an original dataset announced via ProteomeXchange.

Title	Temporal landscape of mitochondrial proteostasis governed by the UPRmt
Description	When mitochondrial proteostasis breaks down, quality control pathways including the UPRmt and PINK1/Parkin mitophagy are activated in response. However, beyond the upregulation of chaperones and proteases, we have a limited understanding of how the UPRmt remodels and restores damaged mito-proteomes. Here, we have developed a functional proteomics framework, termed MitoPQ (Mitochondrial Proteostasis Quantification), to dissect the UPRmt’s role in maintaining proteostasis during stress. We discover essential roles for the UPRmt in both protecting and repairing proteostasis, with oxidative phosphorylation metabolism being a central target of the UPRmt. Transcriptome analyses together with MitoPQ reveal that UPRmt transcription factors drive independent signaling arms that act in concert to maintain proteostasis. Unidirectional interplay between the UPRmt and PINK1/Parkin mitophagy was found to promote oxidative phosphorylation recovery when the UPRmt failed. Collectively, this study defines the network of proteostasis mediated by the UPRmt and highlights the value of functional proteomics in decoding stressed proteomes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:01.609.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Steele
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 04:13:37	ID requested
1	2023-09-26 23:55:53	announced
⏵ 2	2023-11-14 08:24:02	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: mitochondria

metabolism

OXPHOS

proteostasis

UPRmt

mitophagy

PINK1

Parkin

Michael Lazarou
contact affiliation	Walter and Eliza Hall Institute of Medical Research, co-affiliated with Monash University
contact email	m.lazarou@wehi.edu.au
lab head
Joel Steele
contact affiliation	Monash
contact email	joel.steele@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD038403

PRIDE project URI

• PRIDE
  1. PXD038403
     1. Label: PRIDE project
     2. Name: Temporal landscape of mitochondrial proteostasis governed by the UPRmt