PXD038398

PXD038398 is an original dataset announced via ProteomeXchange.

Title	Translation-coupled sensing and degradation of RNA-protein crosslinks
Description	Reactive aldehydes are abundant cytotoxic metabolites, which challenge homoeostasis by crosslinking cellular macromolecules. Whether RNA damage contributes to the toxicity of aldehydes and whether cells possess mechanisms to resolve RNA-protein crosslinks (RPCs) in particular is unknown. Studying the specific consequences of aldehyde-induced RNA damage is challenging due to confounding induction of DNA damage. Here, we establish photoactivatable ribonucleosides as a tractable model system to study aldehyde-mimicking RNA damage in the absence of DNA damage. The aim of this phosphoproteome measurement is to elucidate the changes in phosphorylation sites upon RPC induction using the treatment with a photoactivatable-ribonucleoside-enhanced crosslinking (PAR-CL) by incubation with 4-thiouridine (4-SU) and subsequent crosslinking by UVA irradiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-13
AnnouncementXML	Submission_2023-11-13_05:26:28.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PetraBeli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 04:11:31	ID requested
⏵ 1	2023-11-13 05:26:28	announced

Dataset with its publication pending

submitter keyword: formaldehyde, RNA-protein crosslinks, UPS,RNA damage, translational stress

PetraBeli
contact affiliation	IMB Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
PetraBeli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD038398

PRIDE project URI

• PRIDE
  1. PXD038398
     1. Label: PRIDE project
     2. Name: Translation-coupled sensing and degradation of RNA-protein crosslinks