PXD038397

PXD038397 is an original dataset announced via ProteomeXchange.

Title	Shotgun analysis of proteins in NDUFA11 KO and WT HEK 293T cells.
Description	The effect of the depletion of an ETC complex I protein component - NDUFA11 on the proteome in HEK 293T cells was studied. TMT-based relative quantification of protein levels were performed in NDUFA11 KO vs WT cells and in NDUFA11 KO vs WT cells that expressed an EGFP-MAPT (Tau protein) construct. In this dataset relative levels of proteins in total cell extracts and in the soluble fraction (post centrifugation at 125,000 g, at which speed protein aggregates were isolated as pellets) were measured. This dataset is directly related to the dataset PXD031374 (protein aggregates isolated as pellets post centrifugation at 125,000 g).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:51:55.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 04:06:17	ID requested
1	2023-07-03 13:34:07	announced
2	2023-11-14 07:01:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:51:55	announced	2024-10-22: Updated project metadata.

10.1038/S41467-023-39642-8;

submitter keyword: NDUFA11 KO, EGFP-MAPT expression, ETC Complex I, HEK 293T

Prof. Agnieszka Chacinska
contact affiliation	ReMedy International Research Agenda Unit, IMol Polish Academy of Sciences, Warsaw, Poland
contact email	a.chacinska@imol.institute
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD038397

PRIDE project URI

• PRIDE
  1. PXD038397
     1. Label: PRIDE project
     2. Name: Shotgun analysis of proteins in NDUFA11 KO and WT HEK 293T cells.