PXD038393

PXD038393 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative proteomics in a cellular model of human ATXN1(Q82) protein aggregation
Description	Spinocerebellar ataxia type-1 is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1 protein. These expansions are responsible for protein misfolding and self-assembly into intranuclear inclusion bodies (IIBs) that are linked to neuronal death. Here, we describe a nuclear protein aggregation model of pathogenic human ataxin-1. Using an inducible Sleeping Beauty transposon system, we overexpressed ATXN1(Q82) in human mesenchymal stem cells that are resistant to the early cytotoxic effects of the mutant protein. We characterized the proteome of cells containing insoluble polyQ IIBs which gradually occupy the nuclei. In response to their formation, our proteomics analysis reveals a perturbed protein interaction network affecting critical cellular processes and indicates major kinases which may affect polyQ protein aggregation.
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_06:46:10.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 04:04:50	ID requested
⏵ 1	2025-02-21 06:46:10	announced

Publication List

10.1371/JOURNAL.PONE.0319084;

10.1371/JOURNAL.PONE.0319084;

Keyword List

submitter keyword: human, SCA1,polyglutamine, ATXN1

submitter keyword: human, SCA1,polyglutamine, ATXN1

Contact List

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD038393
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD038393

PRIDE project URI

Repository Record List

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