<<< Full experiment listing

PXD038387

PXD038387 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMyelin basic protein fragmentation by engineered human proteasomes with different catalytic phenotypes revealed direct peptide ligands of MS-associated and protective HLA class I molecules
DescriptionProteasomes exist in mammalian cells in multiple combinatorial variants due to the broadly diversified regulatory particles and catalytic subunits exchange. Here, using biotin carboxyl carrier domain of transcarboxylase from Propionibacterium shermanii fused with different proteasome subunits of catalytic and regulatory particles, we report comprehensive characterization of highly homogenous one-step purified human constitutive and immune 20S and 26S/30S proteasomes. Hydrolysis of multiple sclerosis (MS) autoantigen, myelin basic protein (MBP), by engineered human proteasomes with different catalytic phenotypes revealed peptides, which may be directly loaded on the HLA class I. Prediction of affinity of HLA class I to these peptides demonstrated that protective HLA I alleles are high affinity binders, whereas MS-associated alleles tend to have moderate-to-low affinity. Core peptide QDENPVVHFF, originated from the encephalitogenic MBP part, and its deaminated form was shown to be high affinity ligand for the protective HLA A*44, -B*40 and -B*35 alleles, demonstrating complexity of the autoimmune neurodegeneration.
HostingRepositoryPRIDE
AnnounceDate2023-01-13
AnnouncementXMLSubmission_2023-01-13_01:01:46.559.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD038387
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRustamZiganshin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-26 03:56:55ID requested
12023-01-13 01:01:46announced
Publication List
10.6019/PXD038387;
Keyword List
submitter keyword: Human, LC-MSMS, MBP, proteosome
Contact List
AlexeyBelogurov
contact affiliationShemyakin-Ovchinnikov Institute of bioorganic chemistry RAS
contact emailalexey.belogurov.jr@gmail.com
lab head
RustamZiganshin
contact affiliationShemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences
contact emailrustam.ziganshin@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD038387
PRIDE project URI
Repository Record List
[ + ]