PXD038376

PXD038376 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication
Description	Chromatin organization must be maintained during cell proliferation to preserve cellular identity and genome integrity. However, DNA replication results in transient displacement of DNA bound proteins, and it is unclear how they regain access to newly replicated DNA. Using quantitative MS-based proteomics coupled to Nascent Chromatin Capture, we provide time resolved binding kinetics for thousands of proteins behind replisomes of mid-S replicated regions. This shows that most proteins regain access within the first 15 minutes after the passage of the fork. In contrast, 25% of the identified proteins do not, and this delay cannot be inferred from their known function, physicochemical properties, or nuclear abundance. Here, we provide data where we impaired the H3K27me3 restoration to analyse its impact into chromatin restoration. To this aim, we used the EPZ-6438 inhibitor, which blocks the histone methyltransferase EZH2. Two time-points are analysed by NCC along the cell cycle in late replicated regions: nascent chromatin, just after the passage of the fork, and a sample 16 hours later, in late G1 phase. Our data support that H3K27me3 have an important role in chromatin restoration. Data available in this entry are related with the supplementary table 6 from our manuscript.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:50.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanesa Alvarez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-26 03:40:08	ID requested
1	2023-02-02 19:21:30	announced
⏵ 2	2023-11-14 08:59:50	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: TMT,Proteomics

Constance Alabert
contact affiliation	Centre for Gene Regulation and Expression School of Life Sciences, University of Dundee, Dundee
contact email	C.Alabert@dundee.ac.uk
lab head
Vanesa Alvarez
contact affiliation	University of Dundee
contact email	valvarez@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038376
     1. Label: PRIDE project
     2. Name: Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication