PXD038366

PXD038366 is an original dataset announced via ProteomeXchange.

Title	Spatial proteome map of Cryptosporidium parvum sporozoite
Description	Cryptosporidium is a leading cause of diarrheal disease in children and an important contributor to early childhood mortality. The parasite invades intestinal epithelial cells and remodels them extensively including building an elaborate interface structure. How this occurs at the molecular level is largely unknown. Here, we generated a whole-cell spatial proteome of the Cryptosporidium sporozoite using the spatial proteomics technology hyperLOPIT. These data in combination with genetic and cell-biological experimentation enabled the discovery of the Cryptosporidium secreted effector proteome, including a new secretory organelle.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:51.629.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Konstantin Barylyuk
SpeciesList	scientific name: Cryptosporidium parvum Iowa II; NCBI TaxID: 353152; scientific name: Cryptosporidium parvum virus 1; NCBI TaxID: 675060;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-25 07:03:36	ID requested
⏵ 1	2023-11-14 08:59:52	announced

Dataset with its publication pending

submitter keyword: Cryptosporidium, small granules, secretion, SPS-MS3, rhoptry, organelle, TMT, Apicomplexa, hyperLOPIT, spatial proteome, dense granules, effectors, microneme

Ross F. Waller
contact affiliation	Department of Biochemistry, University of Cambridge, Cambridge, UK
contact email	rfw26@cam.ac.uk
lab head
Konstantin Barylyuk
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	kb601@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038366

PRIDE project URI

• PRIDE
  1. PXD038366
     1. Label: PRIDE project
     2. Name: Spatial proteome map of Cryptosporidium parvum sporozoite