PXD038366 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatial proteome map of Cryptosporidium parvum sporozoite |
Description | Cryptosporidium is a leading cause of diarrheal disease in children and an important contributor to early childhood mortality. The parasite invades intestinal epithelial cells and remodels them extensively including building an elaborate interface structure. How this occurs at the molecular level is largely unknown. Here, we generated a whole-cell spatial proteome of the Cryptosporidium sporozoite using the spatial proteomics technology hyperLOPIT. These data in combination with genetic and cell-biological experimentation enabled the discovery of the Cryptosporidium secreted effector proteome, including a new secretory organelle. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:59:51.629.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Konstantin Barylyuk |
SpeciesList | scientific name: Cryptosporidium parvum Iowa II; NCBI TaxID: 353152; scientific name: Cryptosporidium parvum virus 1; NCBI TaxID: 675060; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-25 07:03:36 | ID requested | |
⏵ 1 | 2023-11-14 08:59:52 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cryptosporidium, small granules, secretion, SPS-MS3, rhoptry, organelle, TMT, Apicomplexa, hyperLOPIT, spatial proteome, dense granules, effectors, microneme |
Contact List
Ross F. Waller |
contact affiliation | Department of Biochemistry, University of Cambridge, Cambridge, UK |
contact email | rfw26@cam.ac.uk |
lab head | |
Konstantin Barylyuk |
contact affiliation | Department of Biochemistry, University of Cambridge |
contact email | kb601@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038366 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038366
- Label: PRIDE project
- Name: Spatial proteome map of Cryptosporidium parvum sporozoite