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PXD038354

PXD038354 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Staphylococcus aureus biofilm quantitative proteomics comparative study to observe the global expression of protein DMSO treatment group and radezolid treatment group
Description	S. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Radezolid was added to the drug treated group until a final concentration of 1/8× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.
HostingRepository	iProX
AnnounceDate	2022-11-25
AnnouncementXML	Submission_2022-11-25_00:02:03.430.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cong Wang
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-25 00:01:30	ID requested
⏵ 1	2022-11-25 00:02:03	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: Staphylococcus aureus, radezolid

Contact List

Zhijian Yu
contact affiliation	Huazhong University of Science and Technology Union Shenzhen Hospital
contact email	yuzhijian@163.com
lab head
Cong Wang
contact affiliation	Huazhong University of Science and Technology Union Shenzhen Hospital
contact email	1403375061@qq.com
dataset submitter

Full Dataset Link List

iProX dataset URI