PXD038344

PXD038344 is an original dataset announced via ProteomeXchange.

Title	Tandem Mass Tag-based Quantitative Proteomics Analyses of a Chicken-Original Virulent and Its Attenuated Histomonas meleagridis Strain in China
Description	Histomonas meleagridis can cause histomonosis in poultry. Due to the prohibition of effective drugs, the prevention and treatment of the disease requires new strategies. Questions about its pathogenic mechanisms and virulence factors remain puzzling. To address some of these issues, comparative proteomic analysis of TMT was performed on a virulent strain and its attenuated strain of Chinese chicken-origin . A total of 3494 proteins were identified in the experiment, of which 745 proteins were differentially expressed (fold change ≥1.2 or ≤0.8 and p＜0.05 ), 192 were up-regulated in virulent strain, and 553 were up-regulated in attenuated strain. Gene ontology (GO) analysis revealed that the differentially expressed proteins were mainly involved in organic material metabolic processes, primary metabolic processes and cellular metabolic processes, exerting protein binding functions, and most were distributed in the cytoplasm and organelle. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that these differentially expressed proteins were mainly involved in pathway of Spliceosome, Cytosolic DNA-sensing pathway, Ribosome biogenesis in eukaryotes, RNA transport and Ribosome. In addition, some differentially expressed proteins were quantified by parallel reverse monitoring (PRM) assay to verify the results of TMT analysis. The above results provide some candidate protein-coding genes for further functional verification, which will help to understand the molecular mechanism of pathogenicity and attenuation of H. meleagridis more comprehensively.
HostingRepository	iProX
AnnounceDate	2022-11-23
AnnouncementXML	Submission_2023-04-10_20:17:20.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guang Qiao
SpeciesList	scientific name: Histomonas meleagridis; NCBI TaxID: 135588;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 19:24:27	ID requested
⏵ 1	2023-04-10 20:17:21	announced

Chen QG, Zhang YM, Chen C, Wang S, Li ZF, Hou ZF, Liu DD, Tao JP, Xu JJ, strain in China. Front Vet Sci, 10():1106807(2023) [pubmed]

submitter keyword: Histomonas meleagridis, Virulence, Attenuation, TMT-based quantitative proteomic analysis, parallel reaction monitoring

jinjun Xu
contact affiliation	Yangzhou University College of Veterinary Medicine
contact email	jjxu@yzu.edu.cn
lab head
Guang Qiao
contact affiliation	Yangzhou University College of Veterinary Medicine
contact email	chenqiaoguang@126.com
dataset submitter

iProX dataset URI