PXD038342

PXD038342 is an original dataset announced via ProteomeXchange.

Title	proteomic response of S. aureus with entrectinib treatment
Description	S. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Entrectinib was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.
HostingRepository	iProX
AnnounceDate	2022-11-23
AnnouncementXML	Submission_2023-01-29_19:50:28.305.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shanghong Liu
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 17:21:06	ID requested
1	2022-11-23 17:21:50	announced
⏵ 2	2023-01-29 19:50:29	announced	2023-01-30: Update publication information.

Liu S, Xiong Y, Xiao H, Zheng J, Wen Z, Li D, Deng Q, Yu Z, by entrectinib through disrupting the cell membrane. Front Microbiol, 13():1106319(2022) [pubmed]

submitter keyword: entrectinib, S. aureus

Zhijian Yu
contact affiliation	Shenzhen University
contact email	yuzhijiansmu@163.com
lab head
Shanghong Liu
contact affiliation	Shenzhen University
contact email	2447471096@qq.com
dataset submitter

iProX dataset URI