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PXD038342

PXD038342 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titleproteomic response of S. aureus with entrectinib treatment
DescriptionS. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. Entrectinib was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.
HostingRepositoryiProX
AnnounceDate2022-11-23
AnnouncementXMLSubmission_2022-11-23_17:21:49.805.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShanghong Liu
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-23 17:21:06ID requested
12022-11-23 17:21:50announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: entrectinib, S. aureus
Contact List
Zhijian Yu
contact affiliationShenzhen University
contact emailyuzhijiansmu@163.com
lab head
Shanghong Liu
contact affiliationShenzhen University
contact email2447471096@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI