PXD038334

PXD038334 is an original dataset announced via ProteomeXchange.

Title	A new LD protein, ApoL6 disrupts the Perilipin 1-HSL interaction to inhibit lipolysis
Description	ApoL6 is a new LD-associated protein containing an apoprotein-like domain, expressed mainly in adipose tissue, specifically in adipocytes. ApoL6 expression is low in fasting but induced upon feeding. ApoL6 knockdown results in smaller LD with lower triglyceride (TAG) content in adipocytes, while ApoL6 overexpression causes larger LD with higher TAG content. We show that ApoL6 effect in adipocytes is by inhibition of lipolysis. While ApoL6, Perilipin 1 (Plin1) and HSL can form a complex on LD, C-terminal domain of ApoL6 directly interacts with Plin1, to compete with Plin1 binding to HSL through Plin1 N-terminal domain, thereby keeping HSL in a stand by status. Thus, ApoL6 ablation decreases WAT mass, protecting mice from diet-induced obesity, while adipose overexpression increases WAT mass to bring obesity and insulin resistance with hepatosteatosis, making ApoL6 a potential future target against obesity and diabetes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:08.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhui Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 04:57:28	ID requested
1	2024-05-24 00:54:18	announced
⏵ 2	2024-10-22 06:42:14	announced	2024-10-22: Updated project metadata.

10.1038/s41467-023-44559-3;

Wang Y, Nguyen HP, Xue P, Xie Y, Yi D, Lin F, Dinh J, Viscarra JA, Ibe NU, Duncan RE, Sul HS, ApoL6 associates with lipid droplets and disrupts Perilipin1-HSL interaction to inhibit lipolysis. Nat Commun, 15(1):186(2024) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP)

submitter keyword: lipolysis, obesity,protein-protein interaction

Hei Sook Sul
contact affiliation	Sul Lab, NST, UC Berkeley
contact email	hsul@berkeley.edu
lab head
Yuhui Wang
contact affiliation	UC Berkeley
contact email	yuhui@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD038334

PRIDE project URI

• PRIDE
  1. PXD038334
     1. Label: PRIDE project
     2. Name: A new LD protein, ApoL6 disrupts the Perilipin 1-HSL interaction to inhibit lipolysis