PXD038330 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Infection-induced peripheral mitochondria fission drives ER encapsulations that rescue bioenergetics |
Description | During infection, viruses rely on altering organelle shape, location, and abundance to modulate cellular functions. The prevalent pathogen human cytomegalovirus (HCMV) induces mitochondria fragmentation while paradoxically promoting respiration. Using super-resolution and cryo-electron tomography, we establish that HCMV infection induces peripheral mitochondria fission, the progeny of which form mitochondria-ER encapsulations (MENCs). Proteomics and metabolic assays demonstrate that MENC resident protein PTPIP51 promotes mitochondria health by regulating calcium transfer, membrane potential, and cellular respiration. MENCs stabilize pro-viral mitochondria-mitochondria contacts (MiMiCs) that facilitate the transfer of membrane potential and further increase respiration. Beyond infection, we show that metastatic melanoma cells, which exhibit similar fragmentation/respiration phenotypes, also form PTPIP51-enriched MENCs. Given the prevalence of mitochondria fragmentation in diverse disease states, MENCs can provide an explanation for uncharacterized facets of pathogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-25 |
AnnouncementXML | Submission_2024-07-25_10:02:50.682.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD038330 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Will Hofstadter |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-23 04:55:50 | ID requested | |
⏵ 1 | 2024-07-25 10:02:51 | announced | |
Publication List
Keyword List
submitter keyword: HCMV,Mitochondria, Virus, PTPIP51 |
Contact List
William Hofstadter |
contact affiliation | Princeton Univeristy, Department of Molecular Biology, Cristea Lab |
contact email | wah2@princeton.edu |
lab head | |
Will Hofstadter |
contact affiliation | Princeton University |
contact email | wah2@princeton.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038330
- Label: PRIDE project
- Name: Infection-induced peripheral mitochondria fission drives ER encapsulations that rescue bioenergetics