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PXD038327

PXD038327 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCSE1L and NUP37 proximity labeling in MCF10A cells expressing chimeric ErbB1:ErbB2 receptors
DescriptionThis project uses an MCF10A clone (B2B1) expressing chimeric synthetic receptors for ErbB1 (EGFR) and ErbB2 (HER2) along with BirA*-fused inducible alleles of CSE1L or NUP37. Cells were cultured with reconstituted basement membrane, BirA* alleles induced with doxycycline, ErbB receptors dimerized with a synthetic small molecule, and biotinylation was performed for 24 hours. Lysates underwent a two-step biotin affinity purification before gel electrophoresis and mass spectrometry on selected gel fragments.
HostingRepositoryPRIDE
AnnounceDate2023-03-22
AnnouncementXMLSubmission_2023-03-22_09:05:38.832.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD038327
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKevinJanes
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-23 04:46:40ID requested
12023-03-22 09:05:39announced
Publication List
10.6019/PXD038327;
Keyword List
submitter keyword: MCF-10A, BirA, breast, HER2, BioID, EGFR
Contact List
Kevin AllynJanes
contact affiliationUniversity of Virginia
contact emailkjanes@virginia.edu
lab head
KevinJanes
contact affiliationUniversity of Virginia
contact emailkjanes@virginia.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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