PXD038327

PXD038327 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CSE1L and NUP37 proximity labeling in MCF10A cells expressing chimeric ErbB1:ErbB2 receptors
Description	This project uses an MCF10A clone (B2B1) expressing chimeric synthetic receptors for ErbB1 (EGFR) and ErbB2 (HER2) along with BirA-fused inducible alleles of CSE1L or NUP37. Cells were cultured with reconstituted basement membrane, BirA alleles induced with doxycycline, ErbB receptors dimerized with a synthetic small molecule, and biotinylation was performed for 24 hours. Lysates underwent a two-step biotin affinity purification before gel electrophoresis and mass spectrometry on selected gel fragments.
HostingRepository	PRIDE
AnnounceDate	2023-03-22
AnnouncementXML	Submission_2023-03-22_09:05:38.832.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038327
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	KevinJanes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 04:46:40	ID requested
⏵ 1	2023-03-22 09:05:39	announced

Publication List

10.6019/PXD038327;

10.6019/PXD038327;

Keyword List

submitter keyword: MCF-10A, BirA, breast, HER2, BioID, EGFR

submitter keyword: MCF-10A, BirA, breast, HER2, BioID, EGFR

Contact List

Kevin AllynJanes
contact affiliation	University of Virginia
contact email	kjanes@virginia.edu
lab head
KevinJanes
contact affiliation	University of Virginia
contact email	kjanes@virginia.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038327
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038327

PRIDE project URI

Repository Record List

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