PXD038309

PXD038309 is an original dataset announced via ProteomeXchange.

Title	3-Mercaptopyruvate sulfur transferase is a protein persulfidase
Description	Protein S-persulfidation (P-SSH) is recognized as a common post-translational modification across all domains of life. It occurs under basal conditions and is often observed to be elevated under stress conditions. However, the mechanism(s) by which proteins are persulfidated inside cells have remained unclear. Here we report that 3-mercaptopyruvate sulfur transferase (MPST) engages in direct protein-to-protein transpersulfidation reactions beyond its previously known protein substrates thioredoxin and MOCS3/Uba4, associated with H2S generation and tRNA thiolation, respectively. We observe that depletion of MPST in human cells lowers overall intracellular protein persulfidation levels and identify a subset of proteins whose persulfidation depends on MPST. The predicted involvement of these proteins in the adaptation to stress responses supports the notion that MPST-dependent protein persulfidation promotes cytoprotective functions. The observation of MPST-independent protein persulfidation suggests that other protein persulfidases remain to be identified.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:58.197.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marcin Luzarowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 04:25:13	ID requested
1	2023-05-10 13:43:15	announced
⏵ 2	2023-11-14 08:47:06	announced	2023-11-14: Updated project metadata.

Pedre B, Talwar D, Barayeu U, Schilling D, Luzarowski M, Sokolowski M, Glatt S, Dick TP, 3-Mercaptopyruvate sulfur transferase is a protein persulfidase. Nat Chem Biol, 19(4):507-517(2023) [pubmed]

submitter keyword: cytoprotection, post-translational modification, MPST,S-persulfidation, protein persulfidation

Tobias P. Dick
contact affiliation	Division of Redox Regulation, DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), Heidelberg, Germany Faculty of Biosciences, Heidelberg University, Heidelberg, Germany
contact email	T.Dick@dkfz-heidelberg.de
lab head
Marcin Luzarowski
contact affiliation	University of Heidelberg
contact email	m.luzarowski@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD038309

PRIDE project URI

• PRIDE
  1. PXD038309
     1. Label: PRIDE project
     2. Name: 3-Mercaptopyruvate sulfur transferase is a protein persulfidase