PXD038302

PXD038302 is an original dataset announced via ProteomeXchange.

Title	Proteomics reveals unique identities of human TGF-β-induced and thymus-derived CD4+ regulatory T cells
Description	The CD4+ regulatory T (Treg) cell lineage comprises thymus-derived (t)Treg cells and peripherally induced (p)Treg cells. As a model for Treg cells, studies employ TGF-β-induced (i)Treg cells generated from CD4+ conventional T (Tconv) cells in vitro. Here, we describe the relationship of iTreg cells to tTreg and Tconv cells. Proteomic analysis revealed that iTreg, tTreg and Tconv cell populations each have a unique protein expression pattern. iTreg cells had very limited overlap in protein expression with tTreg cells, regardless of cell activation status and instead shared signaling and metabolic proteins with Tconv cells. tTreg cells had a uniquely modest response to CD3/CD28-mediated stimulation. As a benchmark, we used a previously defined proteomic signature that sets ex vivo naïve and effector phenotype Treg cells apart from Tconv cells and includes unique Treg cell properties (Cuadrado et al., Immunity, 2018). This Treg cell core signature was largely absent in iTreg cells. We also used a proteomic signature that distinguishes ex vivo effector Treg cells from Tconv cells and naïve Treg cells. This effector Treg cell signature was partially present in iTreg cells. In conclusion, iTreg cells are distinct from tTreg cells and share limited features with ex vivo Treg cells at the proteomic level.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:33:24.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Mensink
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 01:57:26	ID requested
1	2022-11-28 02:42:43	announced
⏵ 2	2023-11-14 08:33:25	announced	2023-11-14: Updated project metadata.

Mensink M, Schrama E, Cuadrado E, Amsen D, de Kivit S, Borst J, regulatory T cells. Sci Rep, 12(1):20268(2022) [pubmed]

submitter keyword: Human, tTreg, Tconv, iTreg, conventional T cell, thymus-derived regulatory T cell, induced regulatory T cell, Treg, TGF-beta, regulatory T cell

Jannie Borst
contact affiliation	Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands
contact email	J.G.Borst@lumc.nl
lab head
Mark Mensink
contact affiliation	Department of Immunology, Leiden University Medical Center
contact email	m.mensink@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD038302

PRIDE project URI

• PRIDE
  1. PXD038302
     1. Label: PRIDE project
     2. Name: Proteomics reveals unique identities of human TGF-β-induced and thymus-derived CD4+ regulatory T cells