PXD038299 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells |
Description | To address the limitation associated with degron based systems, we have developed iTAG, a novel synthetic tag based on IMiDs/CELMoDs mechanism of action that improves and addresses the limitations of both PROTAC and previous IMiDs/CeLMoDs based tags. Using structural and sequence analysis, we systematically explored native and chimeric degron containing domains (DCDs) and evaluated their ability to induce degradation. We identified the optimal chimeric iTAG(DCD23 60aa) that elicits robust degradation of targets across cell types and subcellular localisations without exhibiting the well documented “hook effect” of PROTAC-based systems. We showed that iTAG can also induce target degradation by murine CRBN and enabled the exploration of natural neo-substrates that can be degraded by murine CRBN. Hence, the iTAG system constitutes a versatile tool to degrade targets across the human and murine proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:51:06.654.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fernando J. Sialana |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-23 01:38:32 | ID requested | |
1 | 2023-06-27 12:39:42 | announced | |
2 | 2023-11-14 09:01:46 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 05:51:07 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.isci.2023.107059; |
Bouguenina H, Nicolaou S, Le Bihan YV, Bowling EA, Calderon C, Caldwell JJ, Harrington B, Hayes A, McAndrew PC, Mitsopoulos C, Sialana FJ, Scarpino A, Stubbs M, Thapaliya A, Tyagi S, Wang HZ, Wood F, Burke R, Raynaud F, Choudhary J, van Montfort RLM, Sadok A, Westbrook TF, Collins I, Chopra R, iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells. iScience, 26(7):107059(2023) [pubmed] |
Keyword List
submitter keyword: LC-MSMS, iMiDs,targeted protein degradation, PROTACs |
Contact List
Jyoti S. Choudhary |
contact affiliation | Institute of Cancer Research, London |
contact email | jyoti.choudhary@icr.ac.uk |
lab head | |
Fernando J. Sialana |
contact affiliation | Institute of Cancer Research |
contact email | fsialana@yahoo.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD038299 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038299
- Label: PRIDE project
- Name: iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells