PXD038298 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A degron blocking strategy towards improved CRBN recruiting PROTAC selectivity |
Description | The different layers of complexity in PROTAC design have slowed the broad implementation of this novel pharmacological approach. Given the challenges and opportunities of CRL4CRBN hijacking PROTACs we set out to identify and develop broadly implementable strategies to generate PROTACs with improved specificity for their targets. We used structural analysis of known neo-substrate degrons to generate “degron blocked IMiD” analogues and validated that they did not degrade any known neo-substrates using quantitative proteomics. We then applied this strategy to a known BRD9 PROTAC (dBRD9-A) to generate a degron-blocking analogue with a specific degradation profile. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:01:59.477.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fernando J. Sialana |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-23 01:38:32 | ID requested | |
1 | 2023-07-10 10:02:36 | announced | |
⏵ 2 | 2023-11-14 09:02:03 | announced | 2023-11-14: Updated project metadata. |
Publication List
Bouguenina H, Scarpino A, O'Hanlon JA, Warne J, Wang HZ, Wah Hak LC, Sadok A, McAndrew PC, Stubbs M, Pierrat OA, Hahner T, Cabry MP, Le Bihan YV, Mitsopoulos C, Sialana FJ, Roumeliotis TI, Burke R, van Montfort RLM, Choudhari J, Chopra R, Caldwell JJ, Collins I, Recruiting PROTAC Selectivity. Chembiochem, 24(23):e202300351(2023) [pubmed] |
Keyword List
submitter keyword: LC-MSMS,human, PROTACs |
Contact List
Jyoti S. Choudhary |
contact affiliation | Institute of Cancer Research, London |
contact email | jyoti.choudhary@icr.ac.uk |
lab head | |
Fernando J. Sialana |
contact affiliation | Institute of Cancer Research |
contact email | fsialana@yahoo.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD038298 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038298
- Label: PRIDE project
- Name: A degron blocking strategy towards improved CRBN recruiting PROTAC selectivity