PXD038286

PXD038286 is an original dataset announced via ProteomeXchange.

Title	Define the interactome of IBTK by Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods
Description	Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods are powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex, BioID labels and identifies proteins that are in close proximity to the bait, resulting in overlapping yet distinct protein identifications. In order to comprehensively characterize the IBTK interactome networks in cells, we developed a tag workflow which allows for both AP-MS and BioID analysis with a single construct, pcDNA5/FRT vector containing FLAG-BirA-IBTK.
HostingRepository	PRIDE
AnnounceDate	2024-05-10
AnnouncementXML	Submission_2024-05-10_07:07:30.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiru Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-22 04:08:57	ID requested
⏵ 1	2024-05-10 07:07:31	announced

10.7554/ELIFE.92236.2;

submitter keyword: AP-MS,IBTK interactome, BioID

Chenji Wang
contact affiliation	Genetics Department, college of Life Sciences, Fudan University, China
contact email	chenjiwang@fudan.edu.cn
lab head
Huiru Sun
contact affiliation	Fudan University
contact email	21112030011@m.fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD038286

PRIDE project URI

• PRIDE
  1. PXD038286
     1. Label: PRIDE project
     2. Name: Define the interactome of IBTK by Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods