PXD038275

PXD038275 is an original dataset announced via ProteomeXchange.

Title	Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space
Description	Mitochondria are complex organelles with different compartments, each harboring their own protein quality control factors. While the chaperones of the mitochondrial matrix have been well characterized, it is poorly understood which chaperones protect the mitochondrial intermembrane space. We show that cytosolic small heat shock proteins are imported under basal conditions into the mitochondrial intermembrane space, where they operate as molecular chaperones. To identify chaperone substrates with MS/MS, we used a molecular trap variant of HSPB1/Hsp27 (S135F mutant). Through affinity-enrichment co-immunoprecipitation we identified significantly enriched substrates versus GFP-negative control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:05.679.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038275
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sara Dufour
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-22 02:58:51	ID requested
1	2022-12-12 01:42:48	announced
⏵ 2	2023-11-14 09:01:06	announced	2023-11-14: Updated project metadata.

10.6019/PXD038275;

submitter keyword: Charcot-Marie-Tooth (CMT) disease, protein quality control, intermembrane space, small heat shock proteins, mitochondria,Chaperones

Vincent Timmerman
contact affiliation	1. Peripheral Neuropathy Research Group, Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium 2. Laboratory of Neuromuscular Pathology, Institute Born Bunge, Antwerp, Belgium
contact email	Vincent.timmerman@uantwerpen.be
lab head
Sara Dufour
contact affiliation	VIB-UGent
contact email	sara.dufour@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD038275

PRIDE project URI

• PRIDE
  1. PXD038275
     1. Label: PRIDE project
     2. Name: Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space