PXD038257
PXD038257 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Excessive MiR-365b-5p Maturation via N6-methyladenosine Regulates Chondrocyte Apoptosis and Phenotype by NCL/MiR-365b-5p/FHL1 Axis in Osteoarthritis |
| Description | RNA pull-down assays were performed as the protocol of the PureBinding RNA-Protein pull-down Kit (Geneseed, Guangzhou, China). Specific biotinylated probes that hybridize to target pri-miR-365 were obtained from Ribobio. The probes (200pmol) targeting pri-miR-365 or control probes and RNA samples of chondrocytes were added to the pull-down system. After the mixture of RNA and probes was denaturated at 85°C for 5 minutes, and hybridized at room temperature for 2 hours, 100 µl streptavidin-coated magnetic beads were added to the mixture. Non-specifically bound RNAs were removed by hybridization, capture, and washing. The proteins that interacted with pri-miR-365 were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Then, pri-miR-365-interacting bands were subjected to mass spectrometry (Beijing Genomics Institute, Shenzhen, China). The result was gained to establish a proteomic library. |
| HostingRepository | iProX |
| AnnounceDate | 2022-11-21 |
| AnnouncementXML | Submission_2022-11-21_19:29:18.043.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Baoxi Yu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-11-21 19:28:12 | ID requested | |
| ⏵ 1 | 2022-11-21 19:29:18 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: pri-miR-365, osteoarthritis, miR-365b-5p |
Contact List
| Ming Fu | |
|---|---|
| contact affiliation | the First Affiliated Hospital of Sun Yat-sen University |
| contact email | fuming@mail.sysu.edu.cn |
| lab head | |
| Baoxi Yu | |
| contact affiliation | the First Affiliated Hospital of Sun Yat-sen University |
| contact email | yubx5@mail2.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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