PXD038249

PXD038249 is an original dataset announced via ProteomeXchange.

Title	Identification of pH-specific protein expression responses by Campylobacter jejuni strain NCTC 11168
Description	Campylobacter jejuni is one of the major causes of food-borne infections world-wide. The species is strictly host associated and tolerates mild levels of acidity and alkalinity. The ability to survive pH challenges is one of the key aspects of the ability of C. jejuni to survive in food, stomach transit and enables host gastrointestinal tract colonisation. In this study C. jejuni reference strain NCTC 11168 grown within its pH physiological normal growth range (pH 5.8, 7.0 and 8.0,  = ~0.5 h-1) and exposed to pH 4.0 shock for 2 hours. Proteins extracted from biomass were quantified using a combined data dependent and independent acquisition label-free based approach with the aim to identify pH-dependent proteins that respond in a growth phase independent manner. It was discovered that gluconate 2-dehydrogenase GdhAB, NssR-regulated globins Cgb and Ctb, cupin domain protein Cj0761, cytochrome c protein CccC (Cj0037c), and phosphate-binding transporter protein PstB all show acidic pH dependent abundance increases but are not activated by sub-lethal acid shock. Glutamate synthase (GLtBD) and the MfrABC and NapAGL respiratory complexes were induced in cells grown at pH 8.0. The response to pH stress by C. jejuni is to bolster microaerobic respiration and at pH 8.0 this is assisted by accumulation of glutamate the conversion of which could bolster fumarate respiration. Global protein abundance reduction of proteins linked to growth and survival overalls aids cellular energy conservation thus preserving a similar growth rate.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:15.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Wilson
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; NCBI TaxID: 192222;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-21 16:45:10	ID requested
1	2023-04-26 17:53:58	announced
⏵ 2	2023-11-14 08:45:23	announced	2023-11-14: Updated project metadata.

Ramires T, Wilson R, Padilha da Silva W, Bowman JP, Identification of pH-specific protein expression responses by Campylobacter jejuni strain NCTC 11168. Res Microbiol, 174(5):104061(2023) [pubmed]

submitter keyword: Campylobacter jejuni

food safety

pH stress

proteomics

Dr Richard Wilson
contact affiliation	Proteomics facility, Central Science Laboratory, University of Tasmania
contact email	richard.wilson@utas.edu.au
lab head
Richard Wilson
contact affiliation	University of Tasmania
contact email	richard.wilson@utas.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038249
     1. Label: PRIDE project
     2. Name: Identification of pH-specific protein expression responses by Campylobacter jejuni strain NCTC 11168