Updated project metadata. The coccolithophore Emiliania huxleyi was acclimated to growth under three temperatures (17, 23 and 28°C), representing control, sub-optimal and supra-optimal warming respectively. Shotgun proteomic analysis was utilised to examine the molecular mechanisms driving the cellular response of E. huxleyi to warming. This revealed a significant reprogramming of the cellular proteome in-line with existing data relating to the relative sensitivities of phytoplankton photosynthesis and respiration to increasing temperature.