PXD038242

PXD038242 is an original dataset announced via ProteomeXchange.

Title	Quantifying mistranslation from alanine tRNA anticodon variants in S. cerevisiae
Description	Transfer RNAs (tRNAs) maintain translational fidelity through strict charging by their cognate aminoacyl-tRNA synthetase and codon:anticodon base pairing with the mRNA at the ribosome. Mistranslation occurs when an amino acid not specified by the genetic code is incorporated into a protein. Since alanyl-tRNA synthetase uniquely recognizes a G3:U70 base pair in alanine tRNAs and the anticodon plays no role in charging, alanine tRNA variants with anticodon mutations have the potential to mistranslate alanine. Our goal was to quantify mis-incorporation of alanine into proteins in Saccharomyces cerevisiae strains expressing one of 57 different alanine tRNA anticodon variants. Using mass spectrometry, we observed mistranslation for 45 of the variants when expressed on single-copy plasmids.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:44.470.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038242
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Berg
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-21 16:19:19	ID requested
1	2023-10-01 23:53:23	announced
⏵ 2	2023-11-14 09:05:52	announced	2023-11-14: Updated project metadata.

Cozma E, Rao M, Dusick M, Genereaux J, Rodriguez-Mias RA, Vill, é, n J, Brandl CJ, Berg MD, variants. RNA Biol, 20(1):791-804(2023) [pubmed]

submitter keyword: LC-MSMS, mistranslation, tRNA variants,yeast

Judit Villen
contact affiliation	Department of Genome Sciences, University of Washington, Seattle, WA, USA
contact email	jvillen@uw.edu
lab head
Matthew Berg
contact affiliation	Department of Genome Sciences, University of Washington
contact email	mattberg@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038242
     1. Label: PRIDE project
     2. Name: Quantifying mistranslation from alanine tRNA anticodon variants in S. cerevisiae