PXD038229

PXD038229 is an original dataset announced via ProteomeXchange.

Title	First identification of a large set of serine hydrolases by activity-based protein profiling in dibutyl phthalate-exposed zebrafish larvae
Description	Despite the involvement of several serine hydrolases (SHs) in the metabolism of xenobiotics such as dibutyl phthalate (DBP), no study has focused on mapping this enzyme class in zebrafish, a model organism frequently used in ecotoxicology. Here, we survey and identify active SHs in zebrafish larvae and search for biological markers of SH type after exposition to DBP. Zebrafish were exposed to 0, 5, and 100 µg/L DBP from 4 to 120 h post-fertilization. A significant decrease in vitellogenin expression level of about 2-fold compared to the control was found in larvae exposed to 100 µg/L DBP for 120h. The first comprehensive profiling of active SHs in zebrafish proteome was achieved with an activity-based protein profiling (ABPP) approach. Among 49 SHs identified with high confidence, one was the carboxypeptidase ctsa overexpressed in larvae exposed to 100 µg/L DBP for 120h. To the best of our knowledge, this is the first time that a carboxypeptidase has been identified as deregulated following exposure to DBP. The overall results indicate that targeted proteomics approaches such as ABPP can therefore be an asset for understanding the mechanism of action related to xenobiotics in ecotoxicology.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:01.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Salnot
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-21 15:44:36	ID requested
1	2023-03-11 11:59:43	announced
⏵ 2	2023-11-14 08:44:02	announced	2023-11-14: Updated project metadata.

Yedji RS, Sohm B, Salnot V, Guillonneau F, Cossu-Leguille C, Battaglia E, First Identification of a Large Set of Serine Hydrolases by Activity-Based Protein Profiling in Dibutyl Phthalate-Exposed Zebrafish Larvae. Int J Mol Sci, 23(24):(2022) [pubmed]

submitter keyword: proteomics,zebrafish larvae, activity-based protein profiling, serine hydrolases, dibutyl phthalate

Eric Battaglia
contact affiliation	Université de Lorraine, LIEC, UMR7360, Campus Bridoux, Metz, F-57070, France
contact email	eric.battaglia@univ-lorraine.fr
lab head
Virginie Salnot
contact affiliation	3p5-proteom'ic plateform
contact email	virginie.salnot@parisdescartes.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038229

PRIDE project URI

• PRIDE
  1. PXD038229
     1. Label: PRIDE project
     2. Name: First identification of a large set of serine hydrolases by activity-based protein profiling in dibutyl phthalate-exposed zebrafish larvae