PXD038204

PXD038204 is an original dataset announced via ProteomeXchange.

Title	The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation
Description	Translational regulation is of paramount importance for proteome remodeling during stem cell differentiation both at the global and transcript-specific levels. In this study, we characterized translational remodeling during hepatogenic differentiation of induced pluripotent stem cells (iPSCs) by polysome profiling. We demonstrate that protein synthesis increases during exit from pluripotency, and is then globally repressed during later steps of hepatogenic maturation. This global downregulation of translation is accompanied by a decrease in the protein abundance of components of the translation machinery, which involves a global reduction in translational efficiency of terminal oligopyrimidine tract (TOP) mRNA encoding translation-related factors. Despite global translational repression during hepatogenic differentiation, key hepatogenic genes remain efficiently translated, and the translation of several transcripts involved in hepato-specific functions and metabolic maturation are even induced. We conclude that, during hepatogenic differentiation, a global decrease in protein synthesis is accompanied by a specific translational rewiring of hepato-specific transcripts.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:59:20.660.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038204
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-17 09:27:37	ID requested
1	2023-01-04 06:47:38	announced
⏵ 2	2023-11-14 08:59:21	announced	2023-11-14: Updated project metadata.

10.6019/PXD038204;

submitter keyword: Translational regulation, TOP mRNA, Hepatocyte differentiation,Stem cells

Prof Patricia Renard
contact affiliation	University of Namur - Narilis - URBC 61 rue de Bruxelles 5000 Namur Belgium
contact email	patsy.renard@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038204
     1. Label: PRIDE project
     2. Name: The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation