PXD038203

PXD038203 is an original dataset announced via ProteomeXchange.

Title	Deep Protein Arginine Asymmetric Di-Methylation Profiling of Breast Cancer Patient-derived Xenografts
Description	Arginine methylation catalyzed by protein arginine methyltransferases (PRMTs) is a prevalent posttranslational modification that regulates diverse cellular processes. Aberrant expression of type I PRMTs that catalyze asymmetric arginine demethylation (ADMA) is often found in cancer; however, little is known about the ADMA status of substrate proteins in tumors. Using LC-MS/MS along with pan-specific ADMA antibodies, we performed global mapping of ADMA in five patient-derived xenograft (PDX) tumors representing different subtypes of human breast cancer and identified 415 methylated peptides from 213 proteins. Approximately 70% of the putative substrates were validated using peptide arrays in vitro methylated by PRMT1, PRMT4, and PRMT6, among which 48% of substrates varied from estrogen receptor (ER) positive and negative tumors. Comparing with our previously identified ADMA sites from breast cancer cell lines, 75 ADMA sites overlapped between cell lines and PDX tumors. Collectively, this study provides a useful resource to PRMT and breast cancer communities to exploit the functions of PRMT dysregulation during breast cancer progression.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:25:46.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Ma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-17 09:26:43	ID requested
1	2024-01-26 06:48:35	announced
⏵ 2	2024-10-22 06:25:47	announced	2024-10-22: Updated project metadata.

Ma M, Liu F, Miles HN, Kim EJ, Fields L, Xu W, Li L, Proteome-wide Profiling of Asymmetric Dimethylated Arginine in Human Breast Tumors. J Am Soc Mass Spectrom, 34(8):1692-1700(2023) [pubmed]

10.1021/jasms.3c00154;

submitter keyword: breast cancer,PDX, ADMA, LC-MS/MS

lingjun Li
contact affiliation	School of Pharmacy University of Wisconsin 777 Highland Ave. Madison, WI 53705-2222
contact email	lingjun.li@wisc.edu
lab head
Min Ma
contact affiliation	UCSD
contact email	mma58@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038203
     1. Label: PRIDE project
     2. Name: Deep Protein Arginine Asymmetric Di-Methylation Profiling of Breast Cancer Patient-derived Xenografts