PXD038196

PXD038196 is an original dataset announced via ProteomeXchange.

Title	Kidney tissue proteome profiles discriminate between short and long duration of delayed graft function in donation after circulatory death donors
Description	Delayed graft function (DGF) is commonly defined as dialysis in the first week post-transplant. This definition, though readily applicable, is too generic and unable to distinguish between “types� of DGF or time needed to recover function. We aimed to profile biological pathways in DCD kidney donors that correlate with DGF and discriminate different durations. N=30 DCD kidney biopsies were selected from the UK Quality in Organ Donation biobank and stratified according to DGF duration (immediate function, IF n=10; “short-DGF� (1-6 days), SDGF n=10; “long-DGF� (7-22 days), LDGF n=10). Samples were matched for donor and recipient demographics and analysed by label-free quantitative proteomics, yielding identification of N=3,378 proteins. Ingenuity Pathway Analysis on differentially abundant proteins showed that SDGF kidneys presented stress response pathways upregulation, while LDGF presented impaired response to stress, compared to IF. LDGF showed extensive metabolic deficits compared to IF and SDGF. DCD kidneys requiring dialysis only in the first week post-transplant present acute cellular injury at donation, alongside repair pathways upregulation. In contrast, DCD kidneys requiring dialysis longer beyond 7 days present minimal metabolic and antioxidant responses, suggesting current DGF definitions might not be adequate in distinguishing different types of injury in the donor kidneys contributing to DGF.
HostingRepository	PRIDE
AnnounceDate	2024-05-23
AnnouncementXML	Submission_2024-05-23_00:02:41.972.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fischer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-17 07:09:29	ID requested
⏵ 1	2024-05-23 00:02:42	announced

10.1016/j.ekir.2024.02.012;

Lo Faro ML, Rozenberg K, Huang H, Maslau S, Bonham S, Fischer R, Kessler B, Leuvenink H, Sharples E, Lindeman JH, Ploeg R, Kidney Tissue Proteome Profiles in Short Versus Long Duration of Delayed Graft Function - A Pilot Study in Donation After Circulatory Death Donors. Kidney Int Rep, 9(5):1473-1483(2024) [pubmed]

submitter keyword: kidney, graft

Roman Fischer
contact affiliation	Target Discovery Institute, University of Oxford
contact email	roman.fischer@ndm.ox.ac.auk
lab head
Roman Fischer
contact affiliation	University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038196
     1. Label: PRIDE project
     2. Name: Kidney tissue proteome profiles discriminate between short and long duration of delayed graft function in donation after circulatory death donors