PXD038183

PXD038183 is an original dataset announced via ProteomeXchange.

Title	Venom composition and pain-causing toxins of the Australian great carpenter bee Xylocopa aruana
Description	Most species of bee are capable of delivering a defensive sting which is often painful. A solitary lifestyle is the ancestral state of bees and most extant species are solitary, but information on bee venoms comes predominantly from studies on eusocial species. In this study we investigated the venom composition of the Australian great carpenter bee, Xylocopa aruana Ritsema, 1876. We show that the venom is relatively simple, composed mainly of one small amphipathic peptide (XYTX1-Xa1a), with lesser amounts of an apamin homologue (XYTX2-Xa2a) and a venom phospholipase-A2 (PLA2). XYTX1-Xa1a is homologous to, and shares a similar mode-of-action to melittin and the bombilitins, the major components of the venoms of the eusocial Apis mellifera (Western honeybee) and Bombus spp. (bumblebee), respectively. XYTX1-Xa1a and melittin directly activate mammalian sensory neurons and cause spontaneous pain behaviours in vivo, effects which are potentiated in the presence of venom PLA2. The apamin-like peptide XYTX2-Xa2a was a relatively weak blocker of small conductance calcium-activated potassium (KCa) channels and, like A. mellifera apamin and mast cell-degranulating peptide, did not contribute to pain behaviours in mice. While the composition and mode-of-action of the venom of X. aruana are similar to that of A. mellifera, the greater potency, on mammalian sensory neurons, of the major pain-causing component in A. mellifera venom may represent an adaptation to the distinct defensive pressures on eusocial Apidae.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:15.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Schendel
SpeciesList	scientific name: Xylocopa aruana; NCBI TaxID: 135674;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-16 14:49:27	ID requested
1	2023-03-11 10:18:22	announced
⏵ 2	2023-11-14 08:43:16	announced	2023-11-14: Updated project metadata.

Shi N, Szanto TG, He J, Schroeder CI, Walker AA, Deuis JR, Vetter I, Panyi G, King GF, Robinson SD, Venom composition and pain-causing toxins of the Australian great carpenter bee Xylocopa aruana. Sci Rep, 12(1):22168(2022) [pubmed]

submitter keyword: xylopin, xylopinin, Apidae, toxin, apamin, melittin,Hymenoptera, carpenter bee

Dr. Samuel D. Robinson
contact affiliation	Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.
contact email	sam.robinson@uq.edu.au
lab head
Vanessa Schendel
contact affiliation	University of Queensland
contact email	v.schendel@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038183

PRIDE project URI

• PRIDE
  1. PXD038183
     1. Label: PRIDE project
     2. Name: Venom composition and pain-causing toxins of the Australian great carpenter bee Xylocopa aruana