PXD038179

PXD038179 is an original dataset announced via ProteomeXchange.

Title	Decoupling of mRNA and protein expression in aging brains reveals the age-dependent adaptation of specific gene subsets
Description	During aging, changes in gene expression are associated with decline in physical and cognitive abilities. Here, we investigated the connection between changes of mRNA and protein expression in the brain by comparing the transcriptome and proteome of the mouse cortex during aging. Our transcriptomic analysis revealed that aging mainly triggers gene activation in the cortex. We showed that increase of mRNA expression correlates with protein expression, specifically in the anterior cingulate cortex where we also observed an increase of cortical thickness during aging. Genes exhibiting an aging-dependent increase of mRNA and protein levels are involved in sensory perception and immune functions. Our proteomic analysis also identified changes in protein abundance in the aging cortex and highlighted a subset of proteins that were differentially enriched but exhibited stable mRNA levels during aging, implying the contribution of aging-related post transcriptional and post-translational mechanisms. These specific genes were associated with general biological processes such as translation, ribosome assembly and protein degradation, but also important brain functions related to neuroplasticity. By decoupling mRNA and protein expression, we have thus characterized distinct subsets of genes that differentially adjust to cellular aging in the cerebral cortex.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:40.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benoit Laurent
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-16 14:35:49	ID requested
1	2023-03-11 10:55:32	announced
⏵ 2	2023-11-14 08:43:42	announced	2023-11-14: Updated project metadata.

Khatir I, Brunet MA, Meller A, Amiot F, Patel T, Lapointe X, Avila Lopez J, Guilloy N, Castonguay A, Husain MA, Germain JS, Boisvert FM, Plourde M, Roucou X, Laurent B, Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets. Cells, 12(4):(2023) [pubmed]

submitter keyword: proteomics, brain, cortex, mouse,aging, mRNA

Benoit Laurent
contact affiliation	Laurent's Lab, Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences, Cancer Research Institute (PRAC), Univeriste de Sherbrooke, Sherbrooke, QC, Canada
contact email	benoit.laurent@usherbrooke.ca
lab head
Benoit Laurent
contact affiliation	Université de Sherbrooke
contact email	benoit.laurent@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038179
     1. Label: PRIDE project
     2. Name: Decoupling of mRNA and protein expression in aging brains reveals the age-dependent adaptation of specific gene subsets