PXD038169

PXD038169 is an original dataset announced via ProteomeXchange.

Title	Identification of long noncoding RNA DANCR binding proteins
Description	To capture endogenous DANCR transcripts, we designed and synthesized 11 5′ biotinylated 90-mer DNA oligonucleotides antisense that spanned the entire length of the target RNA DANCR. Pull-down proteins were separated by SDS-PAGE gel and stained with Fast Silver Stain Kit (Beyotime, China). Cell lysates were incubated with biotinylated DNA probes (10 pmol) and 50 μL of streptavidin-agarose beads (Invitrogen) for 1 hour. The proteins were precipitated and diluted in 50 μl of digestion buffer (8 M urea, 50 mM Tris-HCl pH 7.8), separated by SDS-PAGE gel electrophoresis and visualized by silver staining using the PAGE Gel Silver Staining Kit (Solarbio, Beijing, China) as the protocol described. Specific bands were excised for proteomics screening by mass spectrometry analysis (Tianjin Novogene Bioinformatic Technology, Tianjin, China).
HostingRepository	iProX
AnnounceDate	2022-11-15
AnnouncementXML	Submission_2023-04-16_21:09:33.826.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized glutamic acid
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-15 20:56:33	ID requested
⏵ 1	2023-04-16 21:09:34	announced

Liu F, Cao Y, Zhang C, Su H, /Smad signaling. FASEB J, 37(5):e22926(2023) [pubmed]

submitter keyword: lncRNA, diabetic kidney diseases

Chun Zhang
contact affiliation	Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	1193110582@qq.com
lab head
Feng Liu
contact affiliation	Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	liufenguro@163.com
dataset submitter

iProX dataset URI