PXD038137

PXD038137 is an original dataset announced via ProteomeXchange.

Title	A differential proteomic approach highlighted differences induced by plant growth promoting bacteria in Solanum lycopersicum treated with Kocuria rhizophila and Streptomyces violaceoruber
Description	During the last decades, the use of plant growth promoting bacteria (PGPB) has been found to increase crop yield and quality and to confer abiotic and biotic stress tolerance. However, until now the PGPB mechanism to enhance plant performances is not clearly defined. Recently, our findings demonstrated that inoculations with both Kocuria rhizophila and Streptomyces violaceoruber, as well as their combination, determined an increase of tomato (Solanum lycopersicum) growth and development. In this study, through an advanced differential proteomic approach on tomato leaves, plant molecular mechanisms affected by both K. rhizophila and S. violaceoruber have been elucidated. To this aim, tomato plants were treated with K. rhizophila and/or Streptomyces violaceoruber cultures and grown on coconut fiber in greenhouse. In particular, PGPB treatments were conducted twice, on seed and after two weeks from the seedling by fertirrigation. Thus, the analyses have been performed at 14 days after sowing (DAS) (T1) and 42 DAS (T2). The results confirmed the growth stimulation ability of K. rhizophila/Streptomyces violaceoruber, showing shoot fresh and dry weight significantly improved at each time sampling. For the early phase (DAS-T1) comparative proteomics analysis of tomato plant leaves, 2 biological replicates were set up for the plants used as control (i.e. not subjected to treatment - samples I1 and I2-control I), 2 biological replicates for plants subjected to treatment with K. rhizophila (samples L1 and L2-treatment L), 2 biological replicates for plants subjected to treatment with S. violaceoruber (samples M1 and M2-treatment M), and 2 biological replicates for plants subjected to treatment with a mix of the two bacterial strains (samples N1 and N2-treatment N), for a total of 8 samples of leaf protein extracts. For the late phase (DAS-T2) comparative proteomics analysis of tomato plant leaves, 2 biological replicates were set up for the plants used as control (i.e. not treated - samples A1 and A2 - control A), 2 biological replicates for plants subjected to treatment with K. rhizophila (samples B1 and B2-treatment B), 2 biological replicates for plants subjected to treatment with S. violaceoruber (samples C1 and C2-treatment C), and 2 biological replicates for plants subjected to treatment with a mix of the two bacterial strains (samples D1 and D2-treatment D), for a total of 8 samples of leaf protein extracts. Proteomic analysis was able to identify 239 and 203 significantly differentially represented proteins (DRPs) at T1 and T2, respectively, comparing PGPB-treated vs. untreated control plants. KEGG Orthology (KO) identified DRP belonging to photosynthesis, biosynthesis of secondary metabolites, and carbon metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-05-02
AnnouncementXML	Submission_2024-05-02_03:16:26.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giovanni Renzone
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-14 04:17:53	ID requested
⏵ 1	2024-05-02 03:16:27	announced

10.1016/j.plaphy.2024.108609;

Mauceri A, Puccio G, Faddetta T, Abbate L, Polito G, Caldiero C, Renzone G, Lo Pinto M, Alibrandi P, Vaccaro E, Abenavoli MR, Scaloni A, Sunseri F, Cavalieri V, Palumbo Piccionello A, Gallo G, Mercati F, Integrated omics approach reveals the molecular pathways activated in tomato by Kocuria rhizophila, a soil plant growth-promoting bacterium. Plant Physiol Biochem, 210():108609(2024) [pubmed]

submitter keyword: proteomics, plant growth promoting bacteria,Tomato

Andrea Scaloni
contact affiliation	Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80055 Portici, Italy
contact email	andrea.scaloni@ispaam.cnr.it
lab head
Giovanni Renzone
contact affiliation	ISPAAM-CNR
contact email	giovanni.renzone@cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038137
     1. Label: PRIDE project
     2. Name: A differential proteomic approach highlighted differences induced by plant growth promoting bacteria in Solanum lycopersicum treated with Kocuria rhizophila and Streptomyces violaceoruber