PXD038132

PXD038132 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic discovery of a human RNA ligase
Description	RNA ligases are present across all forms of life. While enzymatic RNA ligation between 5'-PO4 and 3'-OH termini is prevalent in viruses, fungi, and plants, such RNA ligases are yet to be identified in vertebrates. Here, using a nucleotide-based chemical probe targeting human AMPylated proteome, we have enriched and identified the hitherto uncharacterised human protein chromosome 12 open reading frame 29 (C12orf29) as the first human enzyme promoting RNA ligation between 5'-PO4 and 3'-OH termini. C12orf29 catalyses ATP-dependent RNA ligation via a three-step mechanism, involving tandem auto- and RNA AMPylation. Knock-out of C12ORF29 gene impedes the cellular resilience to oxidative stress featuring concurrent RNA degradation, which suggests a role of C12orf29 in maintaining RNA integrity. This data provides the groundwork for establishing a human RNA repair pathway.
HostingRepository	PRIDE
AnnounceDate	2023-01-27
AnnouncementXML	Submission_2023-01-26_23:56:56.782.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PhilipSaumer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; adenylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-14 02:02:50	ID requested
⏵ 1	2023-01-26 23:56:57	announced

Dataset with its publication pending

submitter keyword: RNA ligation, AMPylation,Proteomics, Post-translational modification

AndreasMarx
contact affiliation	Organic Chemistry/Cellular Chemistry, Department of Chemistry, University of Konstanz
contact email	andreas.marx@uni-konstanz.de
lab head
PhilipSaumer
contact affiliation	University of Konstanz
contact email	philip.saumer@uni-konstanz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038132
     1. Label: PRIDE project
     2. Name: Chemoproteomic discovery of a human RNA ligase