PXD038121

PXD038121 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide profiling using sample multiplexing of a human neuroblastoma SH-SY5Y cell line treated with cannabidiol (CBD) and tetrahydrocannabinol (THC)
Description	Cannabis has been used throughout history for medicinal and recreational purposes. The most notable cannabinoids derived from these plants are cannabidiol (CBD) and tetrahydrocannabinol (THC). Although well studied for their therapeutic effects, and highly debated concerning their recreational use, the underlying mechanisms of their biological effects are poorly defined. Here we used isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We highlighted significantly regulated proteins by each treatment and performed pathway classification and associated protein-protein interaction analysis. Our data suggest that these treatments may result in mitochondrial dysfunction and induce endoplasmic reticulum stress. This dataset can be mined further to investigate the potential role of CBD and THC in various biological and disease contexts and thus provide a foundation for future studies.
HostingRepository	PRIDE
AnnounceDate	2023-12-28
AnnouncementXML	Submission_2023-12-28_11:16:07.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-12 16:16:56	ID requested
⏵ 1	2023-12-28 11:16:08	announced

Abyadeh M, Gupta V, Liu X, Rossio V, Mirzaei M, Cornish J, Paulo JA, Haynes PA, Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC). Proteomes, 11(4):(2023) [pubmed]

10.3390/proteomes11040036;

submitter keyword: TMTpro, endoplasmic reticulum stress, mitochondrial dysfunction,Cannabidiol, unfolded protein response, Delta-9-tetrahydrocannabinol, RTS

Joao A. Paulo
contact affiliation	Harvard Medical School Department of Cell Biology 240 Longwood Ave. Boston, Massachusetts 02115, USA
contact email	joao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD038121

PRIDE project URI

• PRIDE
  1. PXD038121
     1. Label: PRIDE project
     2. Name: Proteome-wide profiling using sample multiplexing of a human neuroblastoma SH-SY5Y cell line treated with cannabidiol (CBD) and tetrahydrocannabinol (THC)