PXD038121 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide profiling using sample multiplexing of a human neuroblastoma SH-SY5Y cell line treated with cannabidiol (CBD) and tetrahydrocannabinol (THC) |
Description | Cannabis has been used throughout history for medicinal and recreational purposes. The most notable cannabinoids derived from these plants are cannabidiol (CBD) and tetrahydrocannabinol (THC). Although well studied for their therapeutic effects, and highly debated concerning their recreational use, the underlying mechanisms of their biological effects are poorly defined. Here we used isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We highlighted significantly regulated proteins by each treatment and performed pathway classification and associated protein-protein interaction analysis. Our data suggest that these treatments may result in mitochondrial dysfunction and induce endoplasmic reticulum stress. This dataset can be mined further to investigate the potential role of CBD and THC in various biological and disease contexts and thus provide a foundation for future studies. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-28 |
AnnouncementXML | Submission_2023-12-28_11:16:07.243.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joao Paulo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-12 16:16:56 | ID requested | |
⏵ 1 | 2023-12-28 11:16:08 | announced | |
Publication List
Abyadeh M, Gupta V, Liu X, Rossio V, Mirzaei M, Cornish J, Paulo JA, Haynes PA, Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC). Proteomes, 11(4):(2023) [pubmed] |
10.3390/proteomes11040036; |
Keyword List
submitter keyword: TMTpro, endoplasmic reticulum stress, mitochondrial dysfunction,Cannabidiol, unfolded protein response, Delta-9-tetrahydrocannabinol, RTS |
Contact List
Joao A. Paulo |
contact affiliation | Harvard Medical School Department of Cell Biology 240 Longwood Ave. Boston, Massachusetts 02115, USA |
contact email | joao_paulo@post.harvard.edu |
lab head | |
Joao Paulo |
contact affiliation | Harvard Medical School |
contact email | joao_paulo@post.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD038121 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038121
- Label: PRIDE project
- Name: Proteome-wide profiling using sample multiplexing of a human neuroblastoma SH-SY5Y cell line treated with cannabidiol (CBD) and tetrahydrocannabinol (THC)