PXD038116 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arabidopsis DMC1 ubiquitination sites identification, LC-MS/MS |
Description | To characterize the mechanism of DMC1 ubiquitination as well as further explore the impact of DMC1’s ubiquitination on meiosis, we attempted to identify the potential DMC1 ubiquitination sites. The in vitro ubiquitination assay using recombinant DMC1 heterologously expressed and purified from E. coli as substrate was conducted. Then mass spectrometry assay was performed to identify the ubiquitination sites of DMC1. Five DMC1 lysine residues (K45, K70, K101, K162, and K290) were identified as potential ubiquitination sites. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:06:23.372.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wanyue Xu |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | ubiquitinylated lysine |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-11 09:53:01 | ID requested | |
1 | 2023-10-24 10:21:28 | announced | |
⏵ 2 | 2023-11-14 09:06:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Xu W, Yu Y, Jing J, Wu Z, Zhang X, You C, Ma H, Copenhaver GP, He Y, Wang Y, mediates degradation of the meiosis-specific recombinase DMC1. Nat Commun, 14(1):5044(2023) [pubmed] |
Keyword List
submitter keyword: DMC1,Arabidopsis, LC-MS/MS |
Contact List
Yingxiang Wang |
contact affiliation | School of Life Sciences, Fudan University, China |
contact email | yx_wang@fudan.edu.cn |
lab head | |
Wanyue Xu |
contact affiliation | Fudan University |
contact email | xwydianehsu@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038116
- Label: PRIDE project
- Name: Arabidopsis DMC1 ubiquitination sites identification, LC-MS/MS