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PXD038116

PXD038116 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis DMC1 ubiquitination sites identification, LC-MS/MS
DescriptionTo characterize the mechanism of DMC1 ubiquitination as well as further explore the impact of DMC1’s ubiquitination on meiosis, we attempted to identify the potential DMC1 ubiquitination sites. The in vitro ubiquitination assay using recombinant DMC1 heterologously expressed and purified from E. coli as substrate was conducted. Then mass spectrometry assay was performed to identify the ubiquitination sites of DMC1. Five DMC1 lysine residues (K45, K70, K101, K162, and K290) were identified as potential ubiquitination sites.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:06:23.372.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWanyue Xu
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListubiquitinylated lysine
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-11 09:53:01ID requested
12023-10-24 10:21:28announced
22023-11-14 09:06:28announced2023-11-14: Updated project metadata.
Publication List
Xu W, Yu Y, Jing J, Wu Z, Zhang X, You C, Ma H, Copenhaver GP, He Y, Wang Y, mediates degradation of the meiosis-specific recombinase DMC1. Nat Commun, 14(1):5044(2023) [pubmed]
Keyword List
submitter keyword: DMC1,Arabidopsis, LC-MS/MS
Contact List
Yingxiang Wang
contact affiliationSchool of Life Sciences, Fudan University, China
contact emailyx_wang@fudan.edu.cn
lab head
Wanyue Xu
contact affiliationFudan University
contact emailxwydianehsu@163.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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