PXD038116

PXD038116 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis DMC1 ubiquitination sites identification, LC-MS/MS
Description	To characterize the mechanism of DMC1 ubiquitination as well as further explore the impact of DMC1’s ubiquitination on meiosis, we attempted to identify the potential DMC1 ubiquitination sites. The in vitro ubiquitination assay using recombinant DMC1 heterologously expressed and purified from E. coli as substrate was conducted. Then mass spectrometry assay was performed to identify the ubiquitination sites of DMC1. Five DMC1 lysine residues (K45, K70, K101, K162, and K290) were identified as potential ubiquitination sites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:23.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanyue Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-11 09:53:01	ID requested
1	2023-10-24 10:21:28	announced
⏵ 2	2023-11-14 09:06:28	announced	2023-11-14: Updated project metadata.

Xu W, Yu Y, Jing J, Wu Z, Zhang X, You C, Ma H, Copenhaver GP, He Y, Wang Y, mediates degradation of the meiosis-specific recombinase DMC1. Nat Commun, 14(1):5044(2023) [pubmed]

submitter keyword: DMC1,Arabidopsis, LC-MS/MS

Yingxiang Wang
contact affiliation	School of Life Sciences, Fudan University, China
contact email	yx_wang@fudan.edu.cn
lab head
Wanyue Xu
contact affiliation	Fudan University
contact email	xwydianehsu@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038116

PRIDE project URI

• PRIDE
  1. PXD038116
     1. Label: PRIDE project
     2. Name: Arabidopsis DMC1 ubiquitination sites identification, LC-MS/MS