PXD038100 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global assessment of protein-protein crosslinking in ex vivo eRIC samples |
Description | Male mice on a homogenous C57BL6/J genetic background were sacrificed at 11 to 13 weeks of age by cervical dislocation. Kidneys were immediately harvested, flash frozen in liquid nitrogen and stored at -80°C until use. Animal handling was in accordance with guidelines approved by the European Molecular Biology Laboratory (EMBL). Organ sections (thickness 30 µm) were prepared in a Cryostat (Leica Biosystems, Leica CM3050 S) set to -20 °C and deposited onto SuperFrost glass slides (Carl Roth, H880); the glass slides were pre-cooled to -20°C in the cryostat. ~10 sections were placed on a glass slide, and a total of around 150 sections were prepared for each sample per mouse. Tissue sections on glass slides were then transferred onto metal plates placed on dry ice. The tissue sections were exposed to 1 J/cm2 of 254 nm UV light in a XL 1500 UV Spectrolinker (Spectronics Corporation) and subjected to eRIC (PMID: 30352994). The proteins captured by eRIC were separated by gel electrophoresis, divided into 7 fractions of defined mass, and identified by MS to determine their distribution across the fractions. Crosslinking to other proteins should shift the signal to higher mass fractions. The analyses were conducted in triplicate. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-02 |
AnnouncementXML | Submission_2023-03-02_05:03:18.108.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | FrankStein |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-11 09:00:51 | ID requested | |
⏵ 1 | 2023-03-02 05:03:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNA interactome capture., RBP, RIC, eRIC,RNA binding protein, RBPome |
Contact List
Matthias W.Hentze |
contact affiliation | European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. |
contact email | hentze@embl.de |
lab head | |
FrankStein |
contact affiliation | EMBL |
contact email | frank.stein@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038100 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038100
- Label: PRIDE project
- Name: Global assessment of protein-protein crosslinking in ex vivo eRIC samples