PXD038080

PXD038080 is an original dataset announced via ProteomeXchange.

Title	The VIR-associated proteins were identified by LC-MS/MS
Description	7-day-old Col-0 seedlings were collected, and 2 g of tissue was ground to a fine powder in liquid nitrogen and homogenized in 2 ml of lysis buffer (50 mM Tris-HCl, pH 7.4, 2.5 mM MgCl2, 100 mM KCl, 0.1% [v/v] Nonidet P-40, 1 μg/ml leupeptin, 1 μg/ml aprotonin, 0.5 mM phenylmethylsulfonyl fluoride, one tablet of Complete proteinase inhibitor [Roche], and 50 units/ml RNase OUT [Invitrogen]). The lysate was incubated on ice for 5 min and centrifuged at 13,000 g for 10 min at 4°C to pellet cell debris. Clarified lysates were subjected to immunoprecipitation with anti-VIR antibody or normal rabbit IgG (#2729, Cell Signaling Technology, USA) bound to protein A agarose (Sigma-Aldrich)
HostingRepository	iProX
AnnounceDate	2022-11-14
AnnouncementXML	Submission_2022-12-13_01:40:10.645.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Man Zhang
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-09 19:58:30	ID requested
1	2022-11-13 22:47:47	announced
⏵ 2	2022-12-13 01:40:11	announced	2022-12-13: Update publication information.

Zhang M, Zeng Y, Peng R, Dong J, Lan Y, Duan S, Chang Z, Ren J, Luo G, Liu B, R, ů, ž, i, č, ka K, Zhao K, Wang HB, Jin HL, -methyladenosine RNA modification regulates photosynthesis during photodamage in plants. Nat Commun, 13(1):7441(2022) [pubmed]

submitter keyword: LC-MS/MS

Honglei Jin
contact affiliation	Guangzhou University of Chinese Medicine
contact email	jinhl@gzucm.edu.cn
lab head
Man Zhang
contact affiliation	Guangzhou University of Chinese Medicine
contact email	manzhang163@163.com
dataset submitter

iProX dataset URI