PXD038057

PXD038057 is an original dataset announced via ProteomeXchange.

Title	KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells
Description	Vascular smooth muscle cells (VSMCs) within atherosclerotic lesions undergo a phenotypic switching in a KLF4-dependent manner. Glycolysis plays important roles in transdifferentiation of somatic cells, however, it is unclear whether and how KLF4 mediates the link between glycolytic switch and VSMCs phenotypic transitions. Here, we show that KLF4 upregulation accompanies VSMCs phenotypic switching in atherosclerotic lesions. KLF4 enhances the metabolic switch to glycolysis through increasing PFKFB3 expression. Inhibiting glycolysis suppresses KLF4-induced VSMCs phenotypic switching, demonstrating that glycolytic shift is required for VSMCs phenotypic switching. Mechanistically, KLF4 upregulates expression of circCTDP1 and eEF1A2, both of which cooperatively promote PFKFB3 expression. TMAO induces glycolytic shift and VSMCs phenotypic switching by upregulating KLF4. Our study indicates that KLF4 mediates the link between glycolytic switch and VSMCs phenotypic transitions, suggesting that a previously unrecognized KLF4-eEF1A2/circCTDP1-PFKFB3 axis plays crucial roles in VSMCs phenotypic switching.
HostingRepository	iProX
AnnounceDate	2022-11-09
AnnouncementXML	Submission_2022-12-13_01:40:34.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinhua Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-08 20:42:47	ID requested
1	2022-11-08 20:43:00	announced
⏵ 2	2022-12-13 01:40:35	announced	2022-12-13: Update publication information.

Zhang X, Zheng B, Zhao L, Shen J, Yang Z, Zhang Y, Fan R, Zhang M, Ma D, Zheng L, Zhao M, Liu H, Wen J, KLF4-PFKFB3-driven glycolysis is essential for phenotypic switching of vascular smooth muscle cells. Commun Biol, 5(1):1332(2022) [pubmed]

submitter keyword: atherosclerosis,smooth muscle cell,glycolysis,TMAO,PFKFB3,eEF1A2,KLF4

Xinhua Zhang
contact affiliation	Hebei Medical University
contact email	18200814@hebmu.edu.cn
lab head
Xinhua Zhang
contact affiliation	Hebei Medical University
contact email	18200814@hebmu.edu.cn
dataset submitter

iProX dataset URI