PXD038054

PXD038054 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Simultaneous proteome localization and turnover analysis reveals spatiotemporal coordinations of unfolded protein response
Description	The functionality of proteins is dependent on their spatial and temporal distributions, neither of which is directly measured by static protein abundance. Here we report a mass spectrometry-based proteomics workflow and data analysis pipeline, named Simultaneous Proteome Localization and Turnover (SPLAT), to concurrently examine the turnover dynamics and subcellular distributions of whole cell proteomes under perturbation. SPLAT builds on prior work in protein turnover measurements and subcellular localization profiling, by combining dynamic stable isotope labeling, differential ultracentrifugation, and kinetic modeling to concurrently measure changes in protein turnover and subcellular localization under perturbation in one experiment.
HostingRepository	jPOST
AnnounceDate	2022-12-31
AnnouncementXML	Submission_2022-12-30_07:00:05.877.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Lau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4); TMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-08 13:22:44	ID requested
⏵ 1	2022-12-30 07:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: protein turnover, spatial proteomics, dynamic SILAC, TMT

submitter keyword: protein turnover, spatial proteomics, dynamic SILAC, TMT

Contact List

Edward Lau
lab head
Edward Lau
contact affiliation	University of Colorado
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001919/