PXD038046

PXD038046 is an original dataset announced via ProteomeXchange.

Title	Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content
Description	The Golgi plays an essential role in protein and lipid glycosylation. Golgi dysfunction is associated with a variety of diseases including cancer, neurodegeneration and cardiovascular disease. We developed a powerful immunoprecipitation technique to isolate the Golgi by tagging TMEM115. Golgi-immunoprecipitation enriched protein markers specific for the organelle with minimal contamination from other subcellular compartments. Using this technique, we characterised the Golgi proteome, metabolome and lipidome. Proteomics analysis confirmed capture of known Golgi proteins and identified additional candidates enriched in the organelle. Metabolomics analysis revealed selective enrichment of uridine-diphosphate (UDP) sugars and their derivatives and validated SLC35A2 as the subcellular transporter for UDP-hexose. Lastly, lipidomics analysis showed that phospholipids including phosphatidylcholine, phosphatidylinositol and phosphatidylserine are the top enriched Golgi lipids. Our Golgi-IP workflow provides a paradigm for multimodal analysis of Golgi molecular content with enhanced subcellular precision.
HostingRepository	PRIDE
AnnounceDate	2023-04-24
AnnouncementXML	Submission_2023-04-24_14:36:56.540.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raja SekharNirujogi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-08 08:06:06	ID requested
⏵ 1	2023-04-24 14:36:56	announced

Dataset with its publication pending

submitter keyword: Golgi-IP, proteomics, metabolomics, lipidomics, Golgi apparatus

Dario RAlessi
contact affiliation	Medical Research Council (MRC) Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
contact email	d.r.alessi@dundee.ac.uk
lab head
Raja SekharNirujogi
contact affiliation	MRC Protein Phosphorylation Unit, university of Dundee
contact email	rnirujogi@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038046
     1. Label: PRIDE project
     2. Name: Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content