PXD038044 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction) - loading control for glycopeptide enrichment |
Description | To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction– loading control pre-enrichment. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids. |
HostingRepository | PRIDE |
AnnounceDate | 2023-03-20 |
AnnouncementXML | Submission_2023-03-20_16:30:51.122.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | JessicaLewis |
SpeciesList | scientific name: Burkholderia cenocepacia K56-2Valvano; NCBI TaxID: 985076; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-08 08:03:15 | ID requested | |
⏵ 1 | 2023-03-20 16:30:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: glycosylation, CRISPR interference, Burkholderia cenocepacia, glycoproteome,Burkholderia cepacia complex, CRISPRi, glycopeptide enrichment |
Contact List
NichollasScott |
contact affiliation | Department of Microbiology and Immunology, The University of Melbourne, Victoria, Australia |
contact email | nichollas.scott@unimelb.edu.au |
lab head | |
JessicaLewis |
contact affiliation | University of Melbourne |
contact email | jessica.lewis1@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD038044 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038044
- Label: PRIDE project
- Name: Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction) - loading control for glycopeptide enrichment